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dntp mix  (TaKaRa)


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    TaKaRa dntp mix
    Dntp Mix, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 8844 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dntp+mixture/dNTP+Mixture/bio_rxiv__64898__2026__05__05__721418-351-16-21
    Average 96 stars, based on 8844 article reviews
    dntp mix - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Isolated island endemic crabs: Fine-scale genetic population structure of an endangered freshwater crab, Geothelphusa miyakoensis
    Article Snippet: 2019).. Therefore, the Ryukyu Islands are often called “the Galapagos of the Orient.” A recent study has shown that a land bridge, named the Okinawa-Miyako Submarine Plateau (OMSP), once connected Okinawa Island in the central Ryukyu Islands with Miyako Island (Miyakojima) in the southern Ryukyus, between 5.53 and 0.27 million years ago (Watanabe et al. 2023).. Many terrestrial species are believed Introduction

    Article Title: Morphological Examination and Molecular Characterization of Theileria spp. From Dairy Cattle in Lingwu, Ningxia, China
    Article Snippet: annulata, Theileria orientalis, Theileria sinensis, Theileria parva, Theileria mutans, Theileria laurotragi and Theileria velifera [2].. Knowledge on the distribution of pathogens can shed light on the prevention and control of diseases.. Previous studies found that there mainly existed three species for Theileria in cattle in China, namely T. annulata, T. orientalis and T. sinensis [18].

    Article Title: Geographical Divergence and Environmental Drivers of the Symbiotic Bacterial Community Structure in a Koelreuteria ‐Feeding Aphid Species Complex
    Article Snippet: .. The PCR amplification reaction mixture of 25 μL contains 2 μL of DNA, 2.5 μL of 10 × LA PCR Buffer II (Mg 2+ plus), 0.5 μL of dNTP mixture (2.5 mM each), 0.5 μL of each forward and reverse primer (10 μM), 0.5 μL of LA Taq enzyme (TaKaRa Bio Inc., Otsu, Japan), and 18.5 μL of ultrapure water. .. PCR amplification is performed using the ProFlexTM PCR system (Applied Biosystems Inc., Waltham, United States) with the following program: initial denaturation at 95°C for 2 min; 35 cycles of denaturation at 95°C for 15 s, annealing at 65°C for 15 s, and extension at 72°C for 23 s; and a final extension at 72°C for 5 min. 5 μL of PCR product was mixed with 1 μL of 6 × Loading Buffer and analyzed by 1% agarose gel electrophoresis.

    Article Title: Functional characterization of chemosensory proteins in Pharsalia antennata with special emphasis on the roles of CSP2 orthologs in pheromone detection.
    Article Snippet: Behaviorally active compounds of longhorned beetles, including sex/aggregation pheromones and plant odorants, have been extensively applied to the integrated pest management.. It is largely unknown, however, how beetle's chemosensory proteins (CSPs) mediate the detection of semiochemicals.. Here, we characterized six antenna-enriched PantCSPs in Pharsalia antennata, revealing the strong binding to two floral scents syringaldehyde and β-ionone (dissociation constant, Ki < 5.5 μM).

    Amplification:

    Article Title: Morphological Examination and Molecular Characterization of Theileria spp. From Dairy Cattle in Lingwu, Ningxia, China
    Article Snippet: annulata, Theileria orientalis, Theileria sinensis, Theileria parva, Theileria mutans, Theileria laurotragi and Theileria velifera [2].. Knowledge on the distribution of pathogens can shed light on the prevention and control of diseases.. Previous studies found that there mainly existed three species for Theileria in cattle in China, namely T. annulata, T. orientalis and T. sinensis [18].

    Article Title: Geographical Divergence and Environmental Drivers of the Symbiotic Bacterial Community Structure in a Koelreuteria ‐Feeding Aphid Species Complex
    Article Snippet: .. The PCR amplification reaction mixture of 25 μL contains 2 μL of DNA, 2.5 μL of 10 × LA PCR Buffer II (Mg 2+ plus), 0.5 μL of dNTP mixture (2.5 mM each), 0.5 μL of each forward and reverse primer (10 μM), 0.5 μL of LA Taq enzyme (TaKaRa Bio Inc., Otsu, Japan), and 18.5 μL of ultrapure water. .. PCR amplification is performed using the ProFlexTM PCR system (Applied Biosystems Inc., Waltham, United States) with the following program: initial denaturation at 95°C for 2 min; 35 cycles of denaturation at 95°C for 15 s, annealing at 65°C for 15 s, and extension at 72°C for 23 s; and a final extension at 72°C for 5 min. 5 μL of PCR product was mixed with 1 μL of 6 × Loading Buffer and analyzed by 1% agarose gel electrophoresis.

    Incubation:

    Article Title: Mutual feedback regulation between Poly(A)-specific ribonuclease (PARN) and cognate microRNAs
    Article Snippet: .. The reaction was transferred immediately to 42°C in a mixture of RNase inhibitor (40 U/μl), 0.1 M DTT, 10 mM dNTP mixture, 5′ PrimeScript RT buffer (Takara), 10 Mm ATP, T4 DNA ligase (6–40 U) followed by 30 min incubation. .. Then, the oligo 18 anchor (200 ng/μl) was added in the reaction at 42°C, and samples were transferred to 12°C for 2 h. The mixture was then transferred to 42°C and was further incubated with PrimeScript Reverse Transcriptase (10 U/μl, Takara) (preheated at 42°C) for 1 h. Reverse transcriptase and T4 ligase were inactivated at 65°C for 20 min. LM-PAT cDNA was used (1–3 μl) as template for amplification by PCR.

    Real-time Polymerase Chain Reaction:

    Article Title: Dual-Caspase-Mediated Apoptosis Underlies Peritoneal Cell-Free DNA Release After PD-Related Peritonitis
    Article Snippet: .. The real-time reaction mix consisted of 4.95 μL of deionized water, 1.25 μL of Eva Green (intercalating double strand (ds) DNA-binding dye) (Biotium, Hayward, CA, USA), 5 μL of magnesium-free real-time PCR buffer (TakaRa, Shiga, Japan), 1.5 μL of magnesium (TakaRa, Shiga, Japan), 0.5 μL of dNTP mixture (TakaRa, Shiga, Japan), 5 μM of each primer, and 0.3 μL of Takara Ex Taq R-PCR (TakaRa, Shiga, Japan). ..

    Reverse Transcription:

    Article Title: Membranes arrest the coarsening of mitochondrial condensates in human cells.
    Article Snippet: .. The manufacturer’s protocol from Thermo Scientific RevertAid Reverse Transcriptase (EP0441) was followed using Oligo(dT)18 as primer for the first strand cDNA synthesis, RNase inhibitor (Invitrogen), and dNTP mixture (TaKaRa). ..

    cDNA Synthesis:

    Article Title: Membranes arrest the coarsening of mitochondrial condensates in human cells.
    Article Snippet: .. The manufacturer’s protocol from Thermo Scientific RevertAid Reverse Transcriptase (EP0441) was followed using Oligo(dT)18 as primer for the first strand cDNA synthesis, RNase inhibitor (Invitrogen), and dNTP mixture (TaKaRa). ..



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